HOME ABOUT CONTACT AVAILABLE ISSUES SUBSCRIBE MEDIA & ADS
LATEST UPDATES » Vol 22, No 11, November 2018 – The Asian Cancer - War on liver cancer       » 2018 Nobel Prize in Physiology or Medicine winners       » Women more prone to selected chemotherapy side effects       » Tibetan fungus could help fight liver cancer       » Standards for TCM decoction to be applied globally       » RMB200 million donation to build Tsinghua University Biomedical Sciences Building      
EYE ON CHINA
Chinese scientists develop new protocols for DNA-free genome editing in wheat
Genome editing based on the CRISPR/Cas9 IVT or RNP can avoid foreign DNA integrated into host genome and reduce off-target effect. By using this protocol, foreign DNA-free genome-edited plants can be obtained in T0 generation.

Due to the simplicity and high-efficiency, the CRISPR/Cas9 has been almost applied in all the organisms for functional analysis and trait improvements. Currently, delivery of the CRISPR/Cas9 reagents into plant cells is still a major bottleneck for further applications.

Recently, researchers from Dr. GAO Caixia's group at the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, developed protocols for DNA-free genome editing in hexaploid wheat by biolistic delivered CRISPR/Cas9 IVT or RNP.

CRISPR/Cas9 components can be delivered to cells in three forms: DNA expression plasmids (DNA), in vitro transcripts (IVTs) and preassembled ribonucleoprotein complexes (RNPs).

Conventional genome editing in plants delivered the CRISPR/Cas9 DNA into plant cells by Agrobacterium or biolistic transformation. However, the integrated CRISPR/Cas9 DNA can constitutively function and increase the off-target effect.

Also, cross or backcross is needed to segregate the CRISPR/Cas9 cassettes. Therefore, delivery methods for CRISPR/Cas9 IVTs or RNPs are urgently needed genome editing in plants.

The whole procedure of GAO's protocol includes preparation of IVTs and RNPs; validation by in vitro cleavage and transient protoplast assay; delivery by biolistic transformation; recovery of mutants and identification of mutants by pooling methods can be accomplished within 9 to 11 weeks.

Genome editing based on the CRISPR/Cas9 IVT or RNP can avoid foreign DNA integrated into host genome and reduce off-target effect. By using this protocol, foreign DNA-free genome-edited plants can be obtained in T0 generation.

Dr. GAO said: "We believe that this protocol is useful to improve the genome editing in plants and accelerate the process of precision breeding."

This work entitled "Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins" was published in Nature Protocols.

This research was supported by grants from the National Key Research and Development Program of China, the National Transgenic Science and Technology Program, the Chinese Academy of Sciences, and National Natural Science Foundation of China.

Click here for the complete issue.

NEWS CRUNCH  
news Asia is the fastest growing region for nutraceuticals
news 2018 Nobel Prize in Physiology or Medicine winners
news Vitafoods Asia expands by 40 per cent in 2018
PR NEWSWIRE  
Asia Pacific Biotech News
EDITORS' CHOICE  
COLUMNS  

APBN Editorial Calendar 2018
January:
Obesity / Outlook for 2018
February:
Searching for the fountain of youth
March:
Women in Science - Making a difference
April:
Digestive health in the 21st century - Trust your guts
May:
Dental health - The root to good health
June:
Cancer - Therapies and strategies for better patient outcomes
July:
Water management - Technologies for biotech and pharmaceutical industries
August:
Regenerative technology - Meat of the future
September:
Doctor Robot - The digital healthcare revolution
October:
Bones / Breast cancer
November:
Liver health / Top science research nations & institutions
December:
AIDS / Breakthrough of the year/Emerging trends
Editorial calendar is subjected to changes.
MAGAZINE TAGS
About Us
Events
Available issues
Editorial Board
Letters to Editor
Instructions to Authors
Advertise with Us
CONTACT
World Scientific Publishing Co. Pte. Ltd.
5 Toh Tuck Link, Singapore 596224
Tel: 65-6466-5775
Fax: 65-6467-7667
» For Editorial Enquiries:
   biotech_edit@wspc.com or Ms Lim Guan Yu
» For Subscriptions, Advertisements &
   Media Partnerships Enquiries:
   biotech_ad@wspc.com
Copyright© 2018 World Scientific Publishing Co Pte Ltd  •  Privacy Policy