Asia Biotech Live
LATEST UPDATES » Vol 24, No 01, January 2020 – Empowering the Patient - Personalization of Diabetes Management       » First of its kind Human Heart-in-a-Jar Model for therapeutic solutions       » China's National Vaccine Tracking System set to be completed in 2020       » China's amended drug administration law kicks in       » Human activity puts Chinese plant biodiversity at risk, study finds       » Contributory effects of each brain cell type in development of Alzheimer's Disease      
Researchers uncover the mechanism behind cancer-causing E.coli toxin setting the path to new preventive measures for colorectal cancer
An inter-disciplinary team of researchers form the Hong Kong University of Science and Technology (HKUST) unravel how a toxin released by E.coli is linked to colorectal cancer through a synthetic biology approach

Escherichia coli (E.coli), a human gut bacterium known to assist in food digestion and regulation of our immune system, also contains toxins that could halt cell cycles and eventually cause cell death. Scientists have long known that colibactin – a genotoxin produced by E.coli can induce DNA mutations in eukaryotic cells and increase the of colorectal cancer in humans. However, the cancer-causing mechanism of colibactin had been a mystery as reconstructing colibactin metabolites is extremely tedious due to the instability of the compound, low titre and complexity of its biosynthetic pathway.

The research team led by Professor Qian Peiyuan, David von Hansemann Professor of Science and Chair Professor of HKUST’s Department of Ocean Science and Division of Life Science, discovered the missing link using a novel biosynthetic method. The team also succeeded in cloning the colibactin gene cluster and found a way to mass produce the genes for testing and validation. After many assays of various sets of colibactin precursors, the team eventually identified colibactin-645 as the cause for DNA mutation and unravelled the colibactin metabolite biosynthetic pathway as well as its mechanism of causing DNA damage.

Prof. Qian said, “Although a few colibactin metabolites have been reported to damage DNA via DNA crosslinking activity, the genotoxic colibactin that possesses DNA double-strands directly is yet-to-be-identified. Our research has confirmed colibactin-645 exerts direct DNA double-strand breaks, that unearthed the missing link that correlates colibactin to its health effects on human beings.”

LI Zhongrui, a researcher of the team, said the restructuring of colibactin’s molecular scaffold provides a model for designing and synthesizing potent DNA cleaving agents – such as synthetic restriction “enzymes” or chemotherapeutic agents.

In collaboration with University of California at Berkeley and Scripps Institution of Oceanography of University of California, San Diego, the research findings were recently published in the prestigious scientific journal Nature Chemistry and the early findings on colibactin were published in another scientific journal Nature Chemical Biology.

Click here for the complete issue.

news Joining Forces to Elevate Asia's Healthcare Industry
news 4th Global Feed Summit draws Feed Producers, Technology Providers, Raw Materials Suppliers to Bangkok for Key Discussions
news 2nd China Pharma Digital Innovation Summit to be held in Shanghai
news 2nd China Healthcare Digital Innovation Summit to be held in Shanghai

About Us
Available issues
Editorial Board
Letters to Editor
Contribute to APBN
Advertise with Us
World Scientific Publishing Co. Pte. Ltd.
5 Toh Tuck Link, Singapore 596224
Tel: 65-6466-5775
Fax: 65-6467-7667
» For Editorial Enquiries:
   biotech_edit@wspc.com or Ms Deborah Seah
» For Subscriptions, Advertisements &
   Media Partnerships Enquiries:
Copyright© 2020 World Scientific Publishing Co Pte Ltd  •  Privacy Policy